Versatile optical microscopy using a reconfigurable hemispherical digital condenser

Sanchari Sen; Luis Molina; Dongyu Cao; Darshan B Desai; Ayrton A Bernussi; Luis Grave de Peralta

doi:10.1364/BOE.6.000658

Versatile optical microscopy using a reconfigurable hemispherical digital condenser

Biomed Opt Express. 2015 Feb 3;6(3):658-67. doi: 10.1364/BOE.6.000658. eCollection 2015 Mar 1.

Authors

Sanchari Sen¹, Luis Molina², Dongyu Cao³, Darshan B Desai³, Ayrton A Bernussi⁴, Luis Grave de Peralta¹

Affiliations

¹ Department of Physics, Texas Tech University, Lubbock, TX 79409, USA ; Nano Tech Center, Texas Tech University, Lubbock, TX 79409, USA.
² LJ Technologies, 1041 E 24 St, Hialeah, Fl 331013, USA.
³ Department of Physics, Texas Tech University, Lubbock, TX 79409, USA.
⁴ Nano Tech Center, Texas Tech University, Lubbock, TX 79409, USA ; Department of Electrical and Computer Engineering, Texas Tech University, Lubbock, TX 79409, USA.

Abstract

We present a computer-controlled hemispherical digital condenser and demonstrate that a single device can be used to implement a variety of both well established and novel optical microscopy techniques. We verified the condenser capabilities by imaging fabricated periodic patterned structures and biological samples.

Keywords: (100.0100) Image processing; (110.2990) Image formation theory; (180.0180) Microscopy; (350.5730) Resolution.