A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples

Xiaoxiang Fu; Xiaohan Wang; Yongliang Cui; Ali Wang; Daowan Lai; Yang Liu; Qing X Li; Baomin Wang; Ligang Zhou

doi:10.1016/j.foodchem.2015.02.068

A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples

Food Chem. 2015 Aug 15:181:140-5. doi: 10.1016/j.foodchem.2015.02.068. Epub 2015 Feb 21.

Authors

Xiaoxiang Fu¹, Xiaohan Wang¹, Yongliang Cui¹, Ali Wang¹, Daowan Lai¹, Yang Liu², Qing X Li³, Baomin Wang⁴, Ligang Zhou⁵

Affiliations

¹ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
² Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
³ Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI 96822, USA.
⁴ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: wbaomin@263.net.
⁵ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: lgzhou@cau.edu.cn.

PMID: 25794732
DOI: 10.1016/j.foodchem.2015.02.068

Abstract

Ustiloxin A, a cyclopeptide mycotoxin, was isolated from the pathogenic fungus Villosiclava virens that causes rice false smut, a worldwide devastating rice disease. A monoclonal antibody (mAb) 2D3G5 was generated with ustiloxin A-bovine serum albumin conjugate. A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. It possessed a median inhibition concentration (IC50) of 13.8 ng/mL and a working range of 2.8-72 ng/mL. The mAb 2D3G5 recognized ustiloxin B with the cross-reactivity as 4%. The average recoveries of ustiloxin A from rice false smut balls and peeled rice samples ranged from 92% to 117% and from 92% to 107%, respectively. Comparison of the concentrations of ustiloxin A in rice false smut balls detected by both icELISA and high performance liquid chromatography-photodiode array detection indicated that the developed icELISA was suitable for detection of ustiloxin A in rice food and feed samples.

Keywords: Mycotoxin; Phytotoxin; Rice false smut; Ustiloxin A; Villosiclava virens; icELISA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
Oryza / chemistry*
Peptides, Cyclic / chemistry*

Substances

Antibodies, Monoclonal
Peptides, Cyclic
ustiloxin A