Use of low-power laser to assist the healing of traumatic wounds in rats

Acta Cir Bras. 2015 Mar;30(3):204-8. doi: 10.1590/S0102-865020150030000007.

Abstract

Purpose: To investigate the morphological aspects of the healing of traumatic wounds in rats using low-power laser.

Methods: Twenty four non isogenic, young adult male Wistar rats (Rattus norvegicus) weighing between 200 and 300 g was used. The animals were randomly distributed into two groups: Control (GC) and Laser (GL), with 12 animals each. After shaving, anesthesia was performed in the dorsal region and then a surgical procedure using a scalpel was carried out to make the traumatic wound. GL received five sessions of laser therapy in consecutive days using the following laser parameters: wavelength 660 nm, power 100 mW, dose 10 J/cm2. The wounds were evaluated through measurement of the area and depth of the wound (MW) and histological analysis (HA).

Results: When comparing the GC with the GL in MW there was a difference in area (p<0.001) and depth (p=0.003) measurement of the wounds in GL. The laser group presented more epithelization than GC (p=0.03). The other histological parameters were similar.

Conclusion: The healing of wounds in rats was improved with the use of the laser.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dose-Response Relationship, Radiation
  • Fibroblasts / radiation effects
  • Low-Level Light Therapy / methods*
  • Male
  • Neovascularization, Physiologic / radiation effects
  • Random Allocation
  • Rats, Wistar
  • Reproducibility of Results
  • Skin / radiation effects
  • Time Factors
  • Treatment Outcome
  • Wound Healing / radiation effects*
  • Wounds and Injuries / pathology
  • Wounds and Injuries / radiotherapy*