Bioorthogonal deprotection on the dendritic cell surface for chemical control of antigen cross-presentation

Angew Chem Int Ed Engl. 2015 May 4;54(19):5628-31. doi: 10.1002/anie.201500301. Epub 2015 Mar 17.

Abstract

The activation of CD8(+) T-cells requires the uptake of exogenous polypeptide antigens and proteolytic processing of these antigens to octamer or nonamer peptides, which are loaded on MHC-I complexes and presented to the T-cell. By using an azide as a bioorthogonal protecting group rather than as a ligation handle, masked antigens were generated-antigens that are not recognized by their cognate T-cell unless they are deprotected on the cell using a Staudinger reduction.

Keywords: Staudinger reduction; antigens; azides; bioorthogonal chemistry; cell recognition.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation / immunology*
  • Antigens / chemistry*
  • Antigens / immunology*
  • Azides / chemistry*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • Cross-Priming / immunology*
  • Dendritic Cells / chemistry*
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Histocompatibility Antigens Class I / chemistry
  • Histocompatibility Antigens Class I / immunology
  • Humans
  • Surface Properties

Substances

  • Antigens
  • Azides
  • Histocompatibility Antigens Class I