Abstract
In the hippocampus of mice lacking the gene for serine racemase (SR), a D-serine synthesizing enzyme, in the CaMKIIα-expressing neurons, we observed a significant decrease in the extracellular concentration of D-serine, a coagonist for the N-methyl-D-aspartate type glutamate receptor (NMDAR), and NMDAR hypofunction as revealed by diminished extracellular taurine concentrations after an intra-hippocampal NMDA infusion when compared to the wild type controls. Therefore, the neuronal SR could regulate the extracellular D-serine signaling responsible for NMDAR activation in the hippocampus.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism
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Extracellular Space / metabolism*
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Glutamic Acid / metabolism
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Glycine / metabolism
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Hippocampus / metabolism*
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Mice, Knockout
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Microdialysis
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N-Methylaspartate / administration & dosage
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N-Methylaspartate / metabolism
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Neurons / enzymology*
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Racemases and Epimerases / genetics
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Racemases and Epimerases / metabolism*
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Receptors, N-Methyl-D-Aspartate / metabolism*
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Serine / metabolism*
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Taurine / metabolism
Substances
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Receptors, N-Methyl-D-Aspartate
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Taurine
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Glutamic Acid
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Serine
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N-Methylaspartate
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Calcium-Calmodulin-Dependent Protein Kinase Type 2
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Camk2a protein, mouse
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Racemases and Epimerases
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serine racemase
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Glycine