DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification

Akihiko Ono; Akira Ito; Taiga Suzuki; Masaki Yamaguchi; Yoshinori Kawabe; Masamichi Kamihira

doi:10.1016/j.jbiosc.2015.02.009

DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification

J Biosci Bioeng. 2015 Oct;120(4):463-6. doi: 10.1016/j.jbiosc.2015.02.009. Epub 2015 Mar 12.

Authors

Akihiko Ono¹, Akira Ito², Taiga Suzuki², Masaki Yamaguchi², Yoshinori Kawabe², Masamichi Kamihira³

Affiliations

¹ Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
² Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
³ Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: kamihira@chem-eng.kyushu-u.ac.jp.

PMID: 25772439
DOI: 10.1016/j.jbiosc.2015.02.009

Abstract

We constructed a DNA damage-responsive transgene expression system mediated by the p53 promoter. We incorporated a transactivation system to generate transcriptional amplification via a positive feedback loop. Higher levels of DNA damage-responsive transgene expression were observed when transactivation was active.

Keywords: DNA damage; Inducible gene expression; Tetracycline transactivator; p53 promoter.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Animals
DNA Damage / drug effects
DNA Damage / genetics*
Feedback, Physiological / drug effects
Humans
Mice
Mutagenicity Tests*
Promoter Regions, Genetic / genetics*
Tetracycline / pharmacology
Transcription, Genetic / drug effects
Transcription, Genetic / genetics*
Transcriptional Activation / drug effects
Transcriptional Activation / genetics*
Transgenes / genetics*
Tumor Suppressor Protein p53 / genetics*
Up-Regulation / drug effects
Up-Regulation / genetics

Substances

TP53 protein, human
Tumor Suppressor Protein p53
Tetracycline