Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples

J Microbiol Methods. 2015 May:112:36-9. doi: 10.1016/j.mimet.2015.03.005. Epub 2015 Mar 11.

Abstract

Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.

Keywords: Diagnostic assays; LAMP; Real-time PCR; Xanthomonas arboricola pv. pruni.

Publication types

  • Evaluation Study
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Microbiological Techniques / methods*
  • Nucleic Acid Amplification Techniques / methods*
  • Plant Diseases / microbiology*
  • Plant Leaves / microbiology
  • Prunus dulcis / microbiology*
  • Sensitivity and Specificity
  • Xanthomonas / genetics
  • Xanthomonas / isolation & purification*