The effect of daily intragastric administration of an aqueous dispersion of silicon nanoparticles (NPs) (the dose range from 1.0 mg/kg to 100 mg/kg body weight for 28 days) to rats on the proteomic profile of liver microsomes has been investigated by 2D-electrophoresis followed by subsequent mass spectrometry identification. The liver microsomal fraction was isolated by differential centrifugation and its protein composition was analyzed by 2D-polyacrylamide gel electrophoresis. Identification of protein spots was carried out using MALDI-TOF mass spectrometric analysis. The mass spectrometry analysis revealed the protein GRP78 (78 kD glucose-regulated protein precursor), belonging to the family of heat shock proteins. This protein present in animals of the control group was not detected in NP-treated rats of group 2 (1 mg/kg body weight/day) and group 3 (10 mg/kg body weight/day). This protein predominantly localized in the liver cell endoplasmic reticulum and plasma membrane has the chaperone biological activity. Possible mechanisms of the effects of engineered nanoparticles on biosynthetic processes in the body are discussed.
Metodom 2D-élektroforeza s posleduiushcheĭ identifikatsieĭ mass-spektra byl izuchen proteomnyĭ profil' mikrosom pecheni krys pod vliianiem ezhednevnogo vnutrizheludochnogo vvedeniia vodnoĭ dispersii nanochastits (NCh) dioksida kremniia v dozakh ot 1,0 do 100 mg/kg massy tela na protiazhenii 28 dneĭ. Mikrosomal'nuiu fraktsiiu vydeliali iz pecheni metodom differentsial'nogo tsentrifugirovaniia. Sostav belkov mikrosomal'noĭ fraktsii analizirovali metodom 2D-élektroforeza v poliakrilamidnom gele. Identifikatsiiu belkovykh piaten osushchestvliali s ispol'zovaniem MALDI-TOF mass-spektrometricheskogo analiza. Macs-spektrometricheskiĭ analiz pozvolil vyiavit' belok GRP78 (78 kD glucose-regulated protein precursor), prinadlezhashchiĭ k semeĭstvu belkov teplovogo shoka, kotoryĭ prisutstvoval v kontrol'noĭ gruppe i ne vyiavlialsia u krys 2-ĭ (1 mg/kg massy tela/den') i 3-ĭ grupp (10 mg/kg massy tela/den') pod vozdeĭstviem NCh. Dannyĭ belok obladaet biologicheskoĭ aktivnost'iu shaperona i lokalizovan preimushchestvenno v éndoplazmaticheskom retikulume i plazmaticheskoĭ membrane kletok pecheni. Obsuzhdaiutsia vozmozhnye mekhanizmy vozdeĭstviia iskusstvennykh nanochastits na biosinteticheskie protsessy v organizme.
Keywords: GRP78; heat shock proteins; nanoparticles; rats; silicon dioxide; two-dimensional electrophoresis.