Inhibitory effect of exendin-4 on secretory group IIA phospholipase A2

Wonhwa Lee; Soyoung Kwak; Hyun-Shik Lee; Dong Hee Na; You-Mie Lee; Jong-Sup Bae

doi:10.1016/j.bbrc.2015.02.165

Inhibitory effect of exendin-4 on secretory group IIA phospholipase A2

Biochem Biophys Res Commun. 2015 Apr 17;459(4):650-4. doi: 10.1016/j.bbrc.2015.02.165. Epub 2015 Mar 7.

Authors

Wonhwa Lee¹, Soyoung Kwak², Hyun-Shik Lee³, Dong Hee Na², You-Mie Lee², Jong-Sup Bae⁴

Affiliations

¹ College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea; Department of Biochemistry and Cell Biology, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
² College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
³ ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Republic of Korea.
⁴ College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: baejs@knu.ac.kr.

PMID: 25757907
DOI: 10.1016/j.bbrc.2015.02.165

Abstract

Exendin-4 (EX4), a glucagon-like peptide-1 receptor agonist, has been reported to attenuate myocardial ischemia and reperfusion injury and inflammatory or oxidative responses. The expression level of secretory group IIA phospholipase A2 (sPLA2-IIA) is elevated in inflammatory diseases. Lipopolysaccharide (LPS) upregulates the expression of sPLA2-IIA in human umbilical vein endothelial cells (HUVECs). Here, EX4 was examined for its effects on the expression and activity of sPLA2-IIA in HUVECs and mice. Pre-treatment of cells or mice with EX4 inhibited LPS-induced sPLA2-IIA expression and activity. Additionally, EX4 suppressed LPS-induced activation of cytosolic phospholipase A2 (cPLA2) and extracellular signal-regulated kinase (ERK) 1/2. Therefore, these results show that EX4 inhibited LPS-induced expression of sPLA2-IIA by suppressing cPLA2 and ERK 1/2.

Keywords: Exendin-4; HUVEC; Inflammation; sPLA2-IIA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Exenatide
Human Umbilical Vein Endothelial Cells
Humans
Male
Mice
Mice, Inbred C57BL
Peptides / physiology*
Phospholipases A2
Venoms

Substances

Peptides
Venoms
Exenatide
Phospholipases A2