Identification of minimum Rpn4-responsive elements in genes related to proteasome functions

FEBS Lett. 2015 Apr 2;589(8):933-40. doi: 10.1016/j.febslet.2015.02.025. Epub 2015 Mar 3.

Abstract

The proteasome is an essential, 66-subunit protease that mediates ubiquitin-dependent proteolysis. The transcription factor Rpn4 regulates concerted expression of proteasome subunits to increase the proteasome by recognizing nonamer proteasome-associated control element (PACE) elements on the promoter regions. However, the genes for proteasome assembly chaperones and some of the subunits have no PACEs. Here we identified a minimal hexamer "PACE-core" sequence that responds to Rpn4. PACE-cores are found in many genes related to proteasome function including the assembly chaperones, but cannot substitute for PACE of the subunits. Our results add a new layer of complexity in transcriptional regulation of genes involved in protein degradation.

Keywords: Proteasome; Rpn4; Transcriptional regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / metabolism*
  • Genes, Fungal / genetics*
  • Proteasome Endopeptidase Complex / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Stress, Physiological / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation

Substances

  • DNA-Binding Proteins
  • RPN4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Proteasome Endopeptidase Complex