The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response

Mem Inst Oswaldo Cruz. 2015 Feb;110(1):134-7. doi: 10.1590/0074-02760140276. Epub 2015 Jan 23.

Abstract

We studied the feasibility of using halloysite clay nanotubes (HNTs) and carboxyl-functionalised multi-walled carbon nanotubes (COOH-MWCNTs) as antigen carriers to improve immune responses against a recombinant LipL32 protein (rLipL32). Immunisation using the HNTs or COOH-MWCNTs significantly increased the rLipL32-specific IgG antibody titres (p < 0.05) of Golden Syrian hamsters. None of the vaccines tested conferred protection against a challenge using a virulent Leptospira interrogans strain. These results demonstrated that nanotubes can be used as antigen carriers for delivery in hosts and the induction of a humoral immune response against purified leptospiral antigens used in subunit vaccine preparations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Silicates / immunology*
  • Animals
  • Antigens / administration & dosage
  • Antigens, Bacterial / administration & dosage
  • Antigens, Bacterial / immunology
  • Bacterial Outer Membrane Proteins / immunology*
  • Bacterial Vaccines / immunology*
  • Carbon Dioxide / immunology
  • Clay
  • Cricetinae
  • Feasibility Studies
  • Immunity, Humoral / immunology
  • Immunoglobulin G / analysis*
  • Leptospira interrogans / classification
  • Leptospira interrogans / immunology*
  • Lipoproteins / immunology*
  • Mesocricetus
  • Nanotubes, Carbon*
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / immunology

Substances

  • Aluminum Silicates
  • Antigens
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • Immunoglobulin G
  • LipL32 protein, Leptospira
  • Lipoproteins
  • Nanotubes, Carbon
  • Recombinant Proteins
  • Carbon Dioxide
  • carboxyl radical
  • Clay