A new circular 4935-bp long plasmid pMTB1 has been identified in sequences of the metagenome. The nucleotide sequence of pMTB1 is highly similar to that of plasmids, pME2001 and pME2200, from the methanogenic archaeon Methanothermobacter marburgensis. One of six putative protein-coding genes encodes a protein containing helix-turn-helix and ATP/GTP-binding motifs and, probably, functioning as a replication initiator protein. Homologs of other genes have been found only in the plasmids of M. marburgensis, but their functions are unknown. Comparison of the complete nucleotide sequences of the plasmids pMTB1, pME2001, and pME2200 has revealed that they have a common origin but differ from each other by the presence of several inserts flanked by nearly perfect direct repeats within regions not essential for replication.