Abstract
Cartilage generation and degradation are regulated by miRNAs. Our previous study has shown altered expression of miR-193b in chondrogenic human adipose-derived mesenchymal stem cells (hADSCs). In the current study, we investigated the role of miR-193b in chondrogenesis and cartilage degradation. Luciferase reporter assays showed that miR-193b targeted seed sequences of the TGFB2 and TGFBR3 3'-UTRs. MiR-193b suppressed the expression of early chondrogenic markers in chondrogenic ATDC5 cells, and TNF-alpha expression in IL-1b-induced PMCs. In conclusion, MiR-193b may inhibit early chondrogenesis by targeting TGFB2 and TGFBR3, and may regulate inflammation by repressing TNF-alpha expression in inflamed chondrocytes.
Keywords:
Chondrogenesis; MiR-193b; Osteoarthritis; TGF-beta; TNF-alpha.
Copyright © 2015. Published by Elsevier B.V.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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Adipose Tissue / cytology
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Animals
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Blotting, Western
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Cell Differentiation / drug effects
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Cell Differentiation / genetics
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Cells, Cultured
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Chondrocytes / cytology
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Chondrocytes / metabolism
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Chondrogenesis / genetics*
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Core Binding Factor Alpha 1 Subunit / genetics
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Core Binding Factor Alpha 1 Subunit / metabolism
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Gene Expression
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Humans
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Interleukin-1beta / pharmacology
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / metabolism
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Mice
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MicroRNAs / genetics*
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Phosphorylation
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Proteoglycans / genetics*
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Proteoglycans / metabolism
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RNA Interference
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Receptors, Transforming Growth Factor beta / genetics*
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Receptors, Transforming Growth Factor beta / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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SOX9 Transcription Factor / genetics
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SOX9 Transcription Factor / metabolism
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Signal Transduction / genetics*
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Smad3 Protein / genetics
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Smad3 Protein / metabolism
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Transforming Growth Factor beta2 / genetics*
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Transforming Growth Factor beta2 / metabolism
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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3' Untranslated Regions
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Core Binding Factor Alpha 1 Subunit
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Interleukin-1beta
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MIRN193 microRNA, human
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MicroRNAs
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Proteoglycans
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Receptors, Transforming Growth Factor beta
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SOX9 Transcription Factor
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Smad3 Protein
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Transforming Growth Factor beta2
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Tumor Necrosis Factor-alpha
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betaglycan