Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus

Anaerobe. 2015 Jun:33:85-9. doi: 10.1016/j.anaerobe.2015.02.004. Epub 2015 Feb 26.

Abstract

PCR-primers were designed for identification of strictly anaerobic bacteria of the genus Zymophilus based on genus-specific sequences of the 16S-23S rDNA internal transcribed spacer region. The specificity of the primers was tested against 37 brewery-related non-target microorganisms that could potentially occur in the same brewery specimens. None DNA was amplified from any of the non-Zymophilus strains tested including genera from the same family (Pectinatus, Megasphaera, Selenomonas), showing thus 100% specificity. PCR assay developed in this study allows an extension of the spectra of detected beer spoilage microorganisms in brewery laboratories.

Keywords: Brewing microbiology; PCR; Strictly anaerobic bacteria; Yeast contamination; Zymophilus paucivorans; Zymophilus raffinosivorans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobiosis
  • Bacteria, Anaerobic / classification*
  • Bacteria, Anaerobic / genetics*
  • Bacteria, Anaerobic / metabolism
  • Base Sequence
  • DNA, Intergenic / chemistry
  • DNA, Intergenic / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics*
  • Sensitivity and Specificity
  • Sequence Alignment

Substances

  • DNA, Intergenic
  • RNA, Ribosomal