A label-free electrochemical DNA biosensor based on covalent immobilization of salmonella DNA sequences on the nanoporous glassy carbon electrode

Biosens Bioelectron. 2015 Jul 15:69:100-5. doi: 10.1016/j.bios.2015.02.024. Epub 2015 Feb 16.

Abstract

Herein, an easy and cost-effective approach to the immobilization of probe was performed. The amino modified salmonella ssDNA probe sequence was covalently linked with carboxylic group on the surface of nanoporous glassy carbon electrode to prepare the DNA biosensor. The differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for the determination of salmonella DNA in the concentration ranges of 10-400pM and 1-400pM with limits of detection of 2.1pM and 0.15pM, respectively.

Keywords: Covalent immobilization; DNA biosensor; Label-free detection; Nanoporous glassy carbon electrode; Salmonella.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biosensing Techniques / instrumentation*
  • Carbon / chemistry
  • Conductometry / instrumentation
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / genetics*
  • Equipment Design
  • Equipment Failure Analysis
  • Food Analysis / instrumentation
  • Food Contamination / analysis
  • Glass / chemistry
  • Microelectrodes*
  • Molecular Sequence Data
  • Nanopores / ultrastructure*
  • Reproducibility of Results
  • Salmonella / genetics*
  • Salmonella / isolation & purification
  • Sensitivity and Specificity
  • Staining and Labeling

Substances

  • DNA, Bacterial
  • Carbon