Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers

Mingqing Huang; Yiping Zhang; Shuyu Xu; Wen Xu; Kedan Chu; Wei Xu; Haiyu Zhao; Jinjian Lu

doi:10.1016/j.jpba.2015.01.049

Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers

J Pharm Biomed Anal. 2015 Apr 10:108:11-20. doi: 10.1016/j.jpba.2015.01.049. Epub 2015 Feb 7.

Authors

Mingqing Huang¹, Yiping Zhang², Shuyu Xu³, Wen Xu³, Kedan Chu³, Wei Xu⁴, Haiyu Zhao⁵, Jinjian Lu⁶

Affiliations

¹ College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. Electronic address: hmq1115@126.com.
² Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
³ College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
⁴ College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. Electronic address: xwfjtcm@sina.com.
⁵ Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100069, China.
⁶ State Key Laboratory for Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomás Pereira Taipa, Macau, China.

PMID: 25703235
DOI: 10.1016/j.jpba.2015.01.049

Abstract

A high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry method was developed for the identification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. A total of 31 compounds (10 phenolic acids, 19 flavonoids, and 2 iridoids) were fully or partially identified. Caffeic acid, neochlorogenic acid, cryptochlorogenin acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, schaftoside, isoschaftoside, flavosativaside, vitexin 2"-rhamnoside, and kaempferol 3-(6"-malonylglucoside) were detected for the first time in this plant. In subsequent quantitative analysis, an ultra-performance liquid chromatography combined with triple quadrupole mass spectrometry method was developed for the quantitative analysis of 17 phenolic compounds. All the analytes were detected within 8 min. The limits of detection and quantification were less than 7.251 and 26.454 ng/mL, respectively. The relative standard deviations of intra-day precision, inter-day precision, repeatability, stability, and recovery were less than 2.87%, 3.87%, 4.86%, 4.70%, and 3.61%, respectively. The validated method was applied to assess the quality of different medicinal parts (leaves, seeds, and roots) of V. negundo. The results indicated that chlorogenic acid, agnuside, isochlorogenic acid A, and isochlorogenic acid C might be selected as quantitative markers for the quality control of V. negundo. The contents of the 17 investigated compounds in leaves differed from those in the other parts. Hierarchical cluster analysis suggested that chlorogenic acid, agnuside, vitexin, and schaftoside could be potential chemical markers for the discrimination of different medicinal parts of V. negundo. This qualitative and quantitative analysis of phenolic compounds of V. negundo could provide a new tool for the quality control of this plant or its related remedies.

Keywords: HPLC–QTOF-MS; Identification; Quantification; UPLC–QqQ-MS; Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz..

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Chromatography, High Pressure Liquid / methods*
Limit of Detection
Mass Spectrometry / methods*
Phenols / chemistry
Phenols / isolation & purification*
Quality Control
Reproducibility of Results
Vitex / chemistry*

Substances

Phenols