Clonal and horizontal spread of the blaOXA-232 gene among Enterobacteriaceae in a Korean hospital

Diagn Microbiol Infect Dis. 2015 May;82(1):70-2. doi: 10.1016/j.diagmicrobio.2015.02.001. Epub 2015 Feb 11.

Abstract

All 16 Klebsiella pneumoniae isolates and both Escherichia coli isolates harbored the bla(OXA-232) and bla(CTX-M-15) genes. Furthermore, all 16 K. pneumoniae isolates belonged to a unique pulsed-field gel electrophoresis clone and were assigned to an identical sequence type (ST14). The 2 E. coli isolates were identified as ST131 and ST457. The bla(OXA-232) gene underwent horizontal transfer to E. coli isolates via a conjugative ColE-type plasmid. The introduction of this K. pneumoniae ST14 strain to the Korean hospital was attributed to an index patient who was likely colonized during a prior hospitalization in India.

Keywords: ColE-type plasmid; Klebsiella pneumoniae ST14; OXA-232; OXA-48.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Conjugation, Genetic
  • Cross Infection / microbiology*
  • Electrophoresis, Gel, Pulsed-Field
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology*
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Female
  • Gene Transfer, Horizontal
  • Genotype
  • Hospitals
  • Humans
  • Klebsiella pneumoniae / classification
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / isolation & purification
  • Korea / epidemiology
  • Male
  • Middle Aged
  • Molecular Epidemiology
  • Molecular Typing
  • Plasmids / analysis*
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • beta-Lactamases