Protein Methionine Sulfoxide Dynamics in Arabidopsis thaliana under Oxidative Stress

Mol Cell Proteomics. 2015 May;14(5):1217-29. doi: 10.1074/mcp.M114.043729. Epub 2015 Feb 18.

Abstract

Reactive oxygen species such as hydrogen peroxide can modify proteins via direct oxidation of their sulfur-containing amino acids, cysteine and methionine. Methionine oxidation, studied here, is a reversible posttranslational modification that is emerging as a mechanism by which proteins perceive oxidative stress and function in redox signaling. Identification of proteins with oxidized methionines is the first prerequisite toward understanding the functional effect of methionine oxidation on proteins and the biological processes in which they are involved. Here, we describe a proteome-wide study of in vivo protein-bound methionine oxidation in plants upon oxidative stress using Arabidopsis thaliana catalase 2 knock-out plants as a model system. We identified over 500 sites of oxidation in about 400 proteins and quantified the differences in oxidation between wild-type and catalase 2 knock-out plants. We show that the activity of two plant-specific glutathione S-transferases, GSTF9 and GSTT23, is significantly reduced upon oxidation. And, by sampling over time, we mapped the dynamics of methionine oxidation and gained new insights into this complex and dynamic landscape of a part of the plant proteome that is sculpted by oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / analysis*
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Gene Deletion
  • Glutathione Transferase / analysis*
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Hydrogen Peroxide / metabolism
  • Hydrogen Peroxide / pharmacology
  • Isoenzymes / analysis
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Kinetics
  • Methionine / analogs & derivatives*
  • Methionine / chemistry
  • Methionine / metabolism*
  • Molecular Sequence Annotation
  • Oxidation-Reduction
  • Oxidative Stress
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plants, Genetically Modified
  • Protein Processing, Post-Translational*

Substances

  • Arabidopsis Proteins
  • Isoenzymes
  • Methionine
  • Hydrogen Peroxide
  • CAT2 protein, Arabidopsis
  • GSTF9 protein, Arabidopsis
  • Glutathione Transferase
  • methionine sulfoxide