Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes: identifying fingerprints of glucose-related metabolic pathways

J Biomed Opt. 2015 May;20(5):051033. doi: 10.1117/1.JBO.20.5.051033.

Abstract

We present the results of a native fluorescence spectroscopy study of blood plasma of rats with experimental diabetes. It was shown that the fluorescence emission band shape at 320 nm excitation is the most indicative of hyperglycemia in the blood plasma samples. We provide the interpretation of this fact based on the changes in reduced nicotinamide adenine dinucleotide phosphate concentration due to glucose-related metabolic pathways and protein fluorescent cross-linking formation following nonenzymatic glycation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / chemistry
  • Body Weight
  • Cross-Linking Reagents / chemistry
  • Diabetes Mellitus, Experimental / blood*
  • Fluorescence
  • Gene Expression Regulation
  • Glucose / chemistry*
  • Glycosylation
  • Hyperglycemia / blood
  • Hyperglycemia / drug therapy*
  • Male
  • Metabolic Networks and Pathways
  • NAD / chemistry
  • NADP / chemistry
  • Plasma / chemistry*
  • Rats
  • Rats, Wistar
  • Spectrometry, Fluorescence / instrumentation*
  • Spectrometry, Fluorescence / methods*

Substances

  • Blood Glucose
  • Cross-Linking Reagents
  • NAD
  • NADP
  • Glucose