Characterization of the aspects of osteoprogenitor cell interactions with physical tetracalcium phosphate anchorage on titanium implant surfaces

Chia-Ling Ko; Ya-Yuan Chang; Cian-Hua Liou; Wen-Cheng Chen

doi:10.1016/j.msec.2014.12.063

Characterization of the aspects of osteoprogenitor cell interactions with physical tetracalcium phosphate anchorage on titanium implant surfaces

Mater Sci Eng C Mater Biol Appl. 2015 Apr:49:7-13. doi: 10.1016/j.msec.2014.12.063. Epub 2014 Dec 19.

Authors

Chia-Ling Ko¹, Ya-Yuan Chang², Cian-Hua Liou², Wen-Cheng Chen³

Affiliations

¹ Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan; Dental Medical Devices and Materials Research Center, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
² Alliance Global Technology Co., Ltd., Kaohsiung Medical Device Special Zone in Southern Taiwan Science Park, Kaohsiung 82151, Taiwan.
³ Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan. Electronic address: wencchen@fcu.edu.tw.

PMID: 25686921
DOI: 10.1016/j.msec.2014.12.063

Abstract

Well-designed implants are used not only to modify the geometry of the implant but also to change the chemical properties of its surfaces. The present study aims to assess the biofunctional effects of tetracalcium phosphate (TTCP) particles as a physical anchor on the implant surface derived through sandblasting. The characteristics of the surface, cell viability, and alkaline phosphatase (ALP) activity toward osteoprogenitor cells (D1) were obtained. D1 cells were cultured on a plain surface that underwent sandblasting and acid etching (SLA) (control SLA group) and on different SLA surfaces with different anchoring TTCP rates (new test groups, M and H). The mean anchoring rates were 57% (M) and 74% (H), and the anchored thickness was estimated to range from 12.6μm to 18.3μm. Compared with the control SLA surface on Ti substrate, the new test groups with different TTCP anchoring rates (M and H) failed to improve cell proliferation significantly but had a well-differentiated D1 cell phenotype that enhanced ALP expression in the early stage of cell cultures, specifically, at day 7. Results suggest that the SLA surface with anchored TTCP can accelerate progenitor bone cell mineralization. This study shows the potential clinical application of the constructed geometry in TTCP anchorage on Ti for dental implant surface modification.

Keywords: Implant; Osseointegration; Progenitor cell; Surface modification; Tetracalcium phosphate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium Phosphates*
Mice
NIH 3T3 Cells
Osteoblasts / cytology*
Stem Cells / cytology*
Surface Properties
Titanium*
X-Ray Diffraction

Substances

Calcium Phosphates
tetracalcium phosphate
Titanium