Complete genome sequence of Pseudomonas fluorescens strain PICF7, an indigenous root endophyte from olive (Olea europaea L.) and effective biocontrol agent against Verticillium dahliae

Pedro Manuel Martínez-García; David Ruano-Rosa; Elisabetta Schilirò; Pilar Prieto; Cayo Ramos; Pablo Rodríguez-Palenzuela; Jesús Mercado-Blanco

doi:10.1186/1944-3277-10-10

Complete genome sequence of Pseudomonas fluorescens strain PICF7, an indigenous root endophyte from olive (Olea europaea L.) and effective biocontrol agent against Verticillium dahliae

Stand Genomic Sci. 2015 Feb 6:10:10. doi: 10.1186/1944-3277-10-10. eCollection 2015.

Authors

Pedro Manuel Martínez-García¹, David Ruano-Rosa², Elisabetta Schilirò², Pilar Prieto³, Cayo Ramos⁴, Pablo Rodríguez-Palenzuela⁵, Jesús Mercado-Blanco²

Affiliations

¹ Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga - Agencia Estatal Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Área de Genética, Facultad de Ciencias, Málaga, Spain ; Centro de Biotecnología y Genómica de Plantas (UPM- INIA), Campus de Montegancedo 28223, Pozuelo de Alarcón, Madrid, Spain.
² Departmentos de Potección de Cultivos y, Campus 'Alameda del Obispo' s/n, Apartado 4084, 14080 Córdoba, Spain.
³ Departmentos de Mejora Genética Vegetal, Instituto de Agricultura Sostenible (CSIC), Campus 'Alameda del Obispo' s/n, Apartado 4084, 14080 Córdoba, Spain.
⁴ Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga - Agencia Estatal Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Área de Genética, Facultad de Ciencias, Málaga, Spain.
⁵ Centro de Biotecnología y Genómica de Plantas (UPM- INIA), Campus de Montegancedo 28223, Pozuelo de Alarcón, Madrid, Spain.

Abstract

Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium is an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, the causal agent of one of the most devastating diseases for olive (Olea europaea L.) cultivation. Here, we announce and describe the complete genome sequence of Pseudomonas fluorescens strain PICF7 consisting of a circular chromosome of 6,136,735 bp that encodes 5,567 protein-coding genes and 88 RNA-only encoding genes. Genome analysis revealed genes predicting factors such as secretion systems, siderophores, detoxifying compounds or volatile components. Further analysis of the genome sequence of PICF7 will help in gaining insights into biocontrol and endophytism.

Keywords: Biocontrol; Detoxification systems; Endophyte; Olive; Pseudomonas fluorescens; Siderophores; Verticillium wilt.