Nucleic acid-mimicking coordination polymer for label-free fluorescent activity assay of histone acetyltransferases

Chem Commun (Camb). 2015 Mar 14;51(21):4469-72. doi: 10.1039/c5cc00067j.

Abstract

A novel and label-free fluorescence assay for histone acetyltransferase (HAT) activity was established via in situ generation of a nucleic acid-mimicking CoA-Au(I) coordination polymer (CP). Moreover, the potency of this assay for HAT-targeted drug discovery was proved by screening HAT inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomimetic Materials / chemistry*
  • Coenzyme A / chemistry
  • Fluorescent Dyes / chemistry*
  • Gold / chemistry
  • Histone Acetyltransferases / metabolism*
  • Nucleic Acids / chemistry
  • Organic Chemicals / chemistry
  • Polymers / chemistry*
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • Nucleic Acids
  • Organic Chemicals
  • Polymers
  • SYBR Green II
  • Gold
  • Histone Acetyltransferases
  • Coenzyme A