Abstract
A chemodosimeter was designed to function as a highly selective phosphate ion (Pi ) sensor, showing a 91-fold ratiometric fluorescence enhancement. The probe successfully visualized exogenous and endogenous apyrase-catalyzed Pi generation and was the first probe able to trace the generation and enrichment of Pi through hemichannel closure in Sf9 cells.
Keywords:
1,8-naphthalene imide; fluorescent probes; in vivo imaging; inorganic phosphate; sensors.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / chemistry*
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Adenosine Triphosphate / metabolism
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Animals
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Apyrase / metabolism
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Caenorhabditis elegans
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Fluorescence
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Ions / analysis
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Ions / metabolism
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Luminescent Measurements
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Molecular Structure
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Naphthalimides / chemical synthesis
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Naphthalimides / chemistry*
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Naphthalimides / metabolism
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Phosphates / analysis*
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Phosphates / metabolism
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Sf9 Cells
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Spodoptera
Substances
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Fluorescent Dyes
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Ions
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Naphthalimides
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Phosphates
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Adenosine Triphosphate
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Apyrase