Highly selective in vivo imaging of endogenous/exogenous phosphate ion over ATP and PPi

Chem Asian J. 2015 May;10(5):1165-9. doi: 10.1002/asia.201500091. Epub 2015 Mar 5.

Abstract

A chemodosimeter was designed to function as a highly selective phosphate ion (Pi ) sensor, showing a 91-fold ratiometric fluorescence enhancement. The probe successfully visualized exogenous and endogenous apyrase-catalyzed Pi generation and was the first probe able to trace the generation and enrichment of Pi through hemichannel closure in Sf9 cells.

Keywords: 1,8-naphthalene imide; fluorescent probes; in vivo imaging; inorganic phosphate; sensors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / chemistry*
  • Adenosine Triphosphate / metabolism
  • Animals
  • Apyrase / metabolism
  • Caenorhabditis elegans
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Ions / analysis
  • Ions / metabolism
  • Luminescent Measurements
  • Molecular Structure
  • Naphthalimides / chemical synthesis
  • Naphthalimides / chemistry*
  • Naphthalimides / metabolism
  • Phosphates / analysis*
  • Phosphates / metabolism
  • Sf9 Cells
  • Spodoptera

Substances

  • Fluorescent Dyes
  • Ions
  • Naphthalimides
  • Phosphates
  • Adenosine Triphosphate
  • Apyrase