Evaluation of phenotypic screening tests for carbapenemase production in Pseudomonas aeruginosa from patients with cystic fibrosis

Anna S Tai; Hanna E Sidjabat; Timothy J Kidd; David M Whiley; David L Paterson; Scott C Bell

doi:10.1016/j.mimet.2015.02.006

Evaluation of phenotypic screening tests for carbapenemase production in Pseudomonas aeruginosa from patients with cystic fibrosis

J Microbiol Methods. 2015 Apr:111:105-7. doi: 10.1016/j.mimet.2015.02.006. Epub 2015 Feb 10.

Authors

Anna S Tai¹, Hanna E Sidjabat², Timothy J Kidd³, David M Whiley⁴, David L Paterson⁵, Scott C Bell⁶

Affiliations

¹ Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia; Adult Cystic Fibrosis Centre, Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, Australia; School of Medicine, The University of Queensland, Brisbane, Australia. Electronic address: anna.tai@uq.net.au.
² University of Queensland, UQ Centre for Clinical Research (UQCCR), Brisbane, Australia. Electronic address: h.sidjabat@uq.edu.au.
³ Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: t.m.kidd@uq.edu.au.
⁴ Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: d.whiley@uq.edu.au.
⁵ University of Queensland, UQ Centre for Clinical Research (UQCCR), Brisbane, Australia. Electronic address: d.paterson1@uq.edu.au.
⁶ Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia; Adult Cystic Fibrosis Centre, Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, Australia; QIMR Berghofer Medical Research Institute, Brisbane, Australia. Electronic address: Scott.Bell@health.qld.gov.au.

PMID: 25680305
DOI: 10.1016/j.mimet.2015.02.006

Abstract

Carbapenemase phenotypic tests (inhibitor-based, modified Hodge and Carba NP) for Pseudomonas aeruginosa isolates from patients with cystic fibrosis (CF) were evaluated with PCR as a reference assay. Except for Carba NP, all methods demonstrated high false positives. Carba NP test presents a promising screening method for CF P. aeruginosa isolates.

Keywords: Carba NP test; Cystic fibrosis; Metallo-β-lactamase; Modified Hodge test; PCR; Pseudomonas aeruginosa.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / metabolism*
Bacteriological Techniques
Cystic Fibrosis / complications
Cystic Fibrosis / microbiology*
False Positive Reactions
Humans
Phenotype
Polymerase Chain Reaction
Pseudomonas Infections / complications
Pseudomonas Infections / microbiology*
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / isolation & purification
beta-Lactamases / metabolism*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase