Progress and challenges in generating functional hematopoietic stem/progenitor cells from human pluripotent stem cells

Senquan Liu; Yulin Xu; Zijing Zhou; Bo Feng; He Huang

doi:10.1016/j.jcyt.2015.01.003

Progress and challenges in generating functional hematopoietic stem/progenitor cells from human pluripotent stem cells

Cytotherapy. 2015 Apr;17(4):344-58. doi: 10.1016/j.jcyt.2015.01.003. Epub 2015 Feb 10.

Authors

Senquan Liu¹, Yulin Xu², Zijing Zhou³, Bo Feng⁴, He Huang⁵

Affiliations

¹ Bone Marrow Transplantation Centre, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
² Bone Marrow Transplantation Centre, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
³ School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
⁴ School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China; SBS Core Laboratory, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China. Electronic address: fengbo@cuhk.edu.hk.
⁵ Bone Marrow Transplantation Centre, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China. Electronic address: hehuangyu@126.com.

PMID: 25680303
DOI: 10.1016/j.jcyt.2015.01.003

Abstract

The generation of hematopoietic stem cells (HSCs) from human pluripotent stem cells (hPSCs) in vitro holds great potential for providing alternative sources of donor cells for clinical HSC transplantation. However, the low efficiency of current protocols for generating blood lineages and the dysfunction identified in hPSC-derived hematopoietic cells limit their use for full hematopoietic reconstitution in clinics. This review outlines the current understanding of in vitro hematopoietic differentiation from hPSCs, emphasizes the intrinsic and extrinsic molecular mechanisms that are attributed to the aberrant phenotype and function in hPSC-derived hematopoietic cells, pinpoints the current challenges to develop the truly functional HSCs from hPSCs for clinical applications and explores their potential solutions.

Keywords: hematopoietic differentiation; hematopoietic reconstitution; hematopoietic stem cells; human embryonic stem cells; induced pluripotent stem cells.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cell Differentiation / genetics
Cell Differentiation / physiology*
Cell Lineage / genetics
Hematopoietic Stem Cells / cytology*
Humans
Mice
Pluripotent Stem Cells / cytology*