A PCR-RFLP method for the simultaneous differentiation of three Entamoeba species

Exp Parasitol. 2015 Apr-May:151-152:80-3. doi: 10.1016/j.exppara.2015.02.003. Epub 2015 Feb 10.

Abstract

Amoebiasis caused by Entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. Despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. The aim of this study was to develop a new diagnostic tool to differentiate E.histolytica from the morphologically undistinguishable E.dispar and E.moshkovskii. We developed a specific, fast and simple PCR-RFLP method that was able to accurately differentiate experimentally-obtained restriction patterns from the three Entamoeba species. This new method could prove useful for clinical and epidemiological purposes.

Keywords: E.dispar; E.moshkovskii; Entamoeba histolytica; PCR-RFLP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxyribonucleases, Type II Site-Specific
  • Diagnosis, Differential
  • Entamoeba / classification*
  • Entamoeba / genetics
  • Entamoeba / isolation & purification
  • Entamoeba histolytica / classification
  • Entamoeba histolytica / genetics
  • Entamoeba histolytica / isolation & purification
  • Entamoebiasis / diagnosis*
  • Entamoebiasis / parasitology
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / standards
  • Polymorphism, Restriction Fragment Length*
  • Restriction Mapping*

Substances

  • endodeoxyribonuclease DdeI
  • Deoxyribonucleases, Type II Site-Specific
  • GANTC-specific type II deoxyribonucleases