Stable and efficient loading of silver nanoparticles in spherical polyelectrolyte brushes and the antibacterial effects

Xiaochi Liu; Yisheng Xu; Xiaohan Wang; Mingfei Shao; Jun Xu; Jie Wang; Li Li; Rui Zhang; Xuhong Guo

doi:10.1016/j.colsurfb.2015.01.040

Stable and efficient loading of silver nanoparticles in spherical polyelectrolyte brushes and the antibacterial effects

Colloids Surf B Biointerfaces. 2015 Mar 1:127:148-54. doi: 10.1016/j.colsurfb.2015.01.040. Epub 2015 Jan 31.

Authors

Xiaochi Liu¹, Yisheng Xu², Xiaohan Wang¹, Mingfei Shao³, Jun Xu¹, Jie Wang¹, Li Li¹, Rui Zhang¹, Xuhong Guo⁴

Affiliations

¹ State-Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.
² State-Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China; Zhejiang Provincial Key Laboratory for Chemical & Biochemical Processing Technology of Farm Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, 318 Liuhe Road, Hangzhou 310023, China. Electronic address: yshxu@ecust.edu.cn.
³ Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, Guangdong 518055, China; Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen, Guangdong 518055, China. Electronic address: mfshao@hitsz.edu.cn.
⁴ State-Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China; Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Shihezi University, Xinjiang 832000, China. Electronic address: guoxuhong@ecust.edu.cn.

PMID: 25677338
DOI: 10.1016/j.colsurfb.2015.01.040

Abstract

A more efficient and convenient strategy was demonstrated to immobilize silver nanoparticles (NPs) with a crystalline structure into the spherical polyelectrolyte brushes (SPB) as an antibacterial material. The SPB used for surface coating (Ag immobilized PVK-PAA SPB) consists of a poly(N-vinylcarbazole) (PVK) core and poly(acrylic acid) (PAA) chain layers which are anchored onto the surface of PVK core at one end. Well-dispersed silver nanoparticles (diameter∼3.5 nm) then formed and were electrostatically confined in the brush layer. Ag content is controlled by a repeated loading process. Thin film coatings were then constructed by layer-by-layer depositions of positive charged poly(diallyldimethylammonium chloride) (PDDA) and SPB. The multilayer composites display excellent stability as well as antibacterial performance but not for simple PVK-PAA coated surface. The results show that almost complete bacteria growth including both dispersed bacterial cells and biofilms was inhibited over a period of 24 h. This approach opens a novel strategy for stable and efficient immobilization of Ag NPs in fabrication of antibacterial materials.

Keywords: Antibacterial; Poly(N-vinylcarbazole); Silver nanoparticles; Spherical polyelectrolyte brush.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry
Anti-Bacterial Agents / pharmacology*
Electrolytes / chemistry*
Escherichia coli / drug effects
Escherichia coli / growth & development
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Microbial Sensitivity Tests
Particle Size
Polyvinyls / chemistry
Silver / chemistry
Silver / pharmacology*
Spectrophotometry, Ultraviolet
Thermogravimetry

Substances

Acrylic Resins
Anti-Bacterial Agents
Electrolytes
Polyvinyls
poly-N-vinylcarbazole
Silver
carbopol 940