Spin-state-selective methods in solution- and solid-state biomolecular 13C NMR

Isabella C Felli; Roberta Pierattelli

doi:10.1016/j.pnmrs.2014.10.001

Spin-state-selective methods in solution- and solid-state biomolecular 13C NMR

Prog Nucl Magn Reson Spectrosc. 2015 Feb:84-85:1-13. doi: 10.1016/j.pnmrs.2014.10.001. Epub 2014 Nov 1.

Authors

Isabella C Felli¹, Roberta Pierattelli²

Affiliations

¹ Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy. Electronic address: felli@cerm.unifi.it.
² Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy. Electronic address: pierattelli@cerm.unifi.it.

PMID: 25669738
DOI: 10.1016/j.pnmrs.2014.10.001

Abstract

Spin-state-selective methods to achieve homonuclear decoupling in the direct acquisition dimension of (13)C detected NMR experiments have been one of the key contributors to converting (13)C detected NMR experiments into really useful tools for studying biomolecules. We discuss here in detail the various methods that have been proposed, summarize the large array of new experiments that have been developed and present applications to different kinds of proteins in different aggregation states.

Keywords: Biomolecules; Homodecoupling; IDP; Paramagnetic molecules; Scalar coupling.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Carbon-13 Magnetic Resonance Spectroscopy / methods*
Nuclear Magnetic Resonance, Biomolecular / methods*