MicroRNA-494 inhibits the growth and angiogenesis-regulating potential of mesenchymal stem cells

FEBS Lett. 2015 Mar 12;589(6):710-7. doi: 10.1016/j.febslet.2015.01.038. Epub 2015 Feb 7.

Abstract

Mesenchymal stem cells (MSCs) play an important role in the pathology of preeclampsia (PE). Our previous microarray analysis found that microRNA-494 (miR-494) is highly expressed in decidua-derived MSCs (dMSCs) from PE. We hypothesized that aberrant expression of miR-494 in dMSCs is involved in PE development. In the present study, we found that miR-494 arrests G1/S transition in dMSCs by targeting CDK6 and CCND1. We also found that supernatant from miR-494-overexpressing dMSCs reduces HTR-8/SVneo migration and impairs HUVEC capillary formation by suppressing VEGF. Taken together, we report an unrecognized mechanism of miR-494 affecting dMSC proliferation and function in the pathology of PE.

Keywords: Angiogenesis; Mesenchymal stem cell; Preeclampsia; Vascular endothelial growth factor; microRNA-494-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Base Sequence
  • Binding Sites
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Decidua / pathology
  • Female
  • G1 Phase Cell Cycle Checkpoints
  • Gene Expression
  • Human Umbilical Vein Endothelial Cells / physiology
  • Humans
  • Mesenchymal Stem Cells / physiology*
  • MicroRNAs / physiology*
  • Neovascularization, Physiologic*
  • Pre-Eclampsia / metabolism
  • Pre-Eclampsia / physiopathology
  • Pregnancy
  • RNA Interference
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • MIRN494 microRNA, human
  • MicroRNAs
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A