Identification of a JAK/STAT pathway receptor domeless from Pacific white shrimp Litopenaeus vannamei

Muting Yan; Chaozheng Li; Ziqi Su; Qianhui Liang; Haoyang Li; Shizhong Liang; Shaoping Weng; Jianguo He; Xiaopeng Xu

doi:10.1016/j.fsi.2015.01.023

Identification of a JAK/STAT pathway receptor domeless from Pacific white shrimp Litopenaeus vannamei

Fish Shellfish Immunol. 2015 May;44(1):26-32. doi: 10.1016/j.fsi.2015.01.023. Epub 2015 Feb 3.

Authors

Muting Yan¹, Chaozheng Li¹, Ziqi Su¹, Qianhui Liang¹, Haoyang Li¹, Shizhong Liang¹, Shaoping Weng¹, Jianguo He², Xiaopeng Xu³

Affiliations

¹ MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
² MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China; School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China. Electronic address: lsshjg@mail.sysu.edu.cn.
³ MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China. Electronic address: xuxpeng@mail.sysu.edu.cn.

PMID: 25659232
DOI: 10.1016/j.fsi.2015.01.023

Abstract

The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway was known to participate in dozens of immune responses in organisms. Domeless, first identified in Drosophila melanogaster, is a unique receptor involved in invertebrate JAK/STAT pathway. In this study, a cytokine receptor (LvDOME) was identified in Litopenaeus vannamei. The LvDOME cDNA was 5178bp in length with an Open Reading Frame (ORF) of 4191bp. LvDOME contained two cytokine binding modules (CBMs) and three fibronectin-type-III-like (FNIII) domains, similar to most vertebrate IL-6 receptors. LvDOME was expressed highest in shrimp muscle and could be up-regulated in the late stage of white spot syndrome virus (WSSV) infection. LvDOME could significantly enhance the activity of the WSSV wsv069 gene promoter through acting on the STAT-binding motif, suggesting LvDOME could activate the JAK/STAT pathway. Moreover, knockdown of LvDOME resulted in lower cumulative mortality of shrimps and less WSSV copies, suggesting LvDOME may be hijacked by WSSV to benefit virus replication. To our knowledge, this is the first report on the receptor of JAK/STAT pathway in shrimp.

Keywords: Domeless; JAK/STAT pathway; Litopenaeus vannamei; LvDOME; WSSV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Arthropod Proteins / chemistry
Arthropod Proteins / genetics*
Arthropod Proteins / metabolism
Gene Expression Regulation
Molecular Sequence Data
Penaeidae / genetics*
Penaeidae / immunology*
Penaeidae / metabolism
Phylogeny
Receptors, Cytokine / chemistry
Receptors, Cytokine / genetics*
Receptors, Cytokine / metabolism
Signal Transduction*
White spot syndrome virus 1 / physiology*

Substances

Arthropod Proteins
Receptors, Cytokine

Associated data

GENBANK/KC346866