Combinatorial therapy using negative pressure and varying lithium dosage for accelerated wound healing

J Mech Behav Biomed Mater. 2015 Apr:44:173-8. doi: 10.1016/j.jmbbm.2015.01.012. Epub 2015 Jan 24.

Abstract

In this work, we investigated the effects of negative pressure, applied using a pump designed for Negative Pressure Wound Therapy (NPWT), on the process of wound healing in vitro via initiation of the Wnt signaling pathway. Results indicate that negative pressure enhanced Wnt signaling and migration into a simulated wound in vitro in NIH-3T3 murine fibroblast cells. Increasing doses of lithium (upto 15 mM) increased basal Wnt signaling and enhanced cell migration into the simulated wound site. A combination of negative pressure and increased doses of lithium synergistically increased Wnt signaling and demonstrated further enhanced cell migration into simulated wound sites, with maximal filling of the simulated wound observed at lithium concentrations of at least 10mM.

Keywords: Migration into score; Murine fibroblasts; Piezoelectric vacuum pump; Wnt signaling; β-catenin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement / drug effects
  • Combined Modality Therapy
  • Dose-Response Relationship, Drug
  • Lithium / pharmacology*
  • Mice
  • NIH 3T3 Cells
  • Negative-Pressure Wound Therapy*
  • Time Factors
  • Transcriptional Activation / drug effects
  • Wnt Signaling Pathway / drug effects
  • Wound Healing / drug effects*
  • beta Catenin / genetics

Substances

  • beta Catenin
  • Lithium