Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury

Junliang Li; Xinke Xu; Shanyi Zhang; Meiguang Zheng; Zhonghua Wu; Yinlun Weng; Leping Ouyang; Jian Yu; Fangcheng Li

doi:10.3969/j.issn.1673-5374.2012.17.009

Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury

Neural Regen Res. 2012 Jun 15;7(17):1338-42. doi: 10.3969/j.issn.1673-5374.2012.17.009.

Authors

Junliang Li¹, Xinke Xu¹, Shanyi Zhang¹, Meiguang Zheng¹, Zhonghua Wu², Yinlun Weng¹, Leping Ouyang¹, Jian Yu¹, Fangcheng Li¹

Affiliations

¹ Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China.
² Department of Neurosurgery, Henan Provincial People's Hospital, Zhengzhou 450003, Henan Province, China.

PMID: 25657665
PMCID: PMC4308805
DOI: 10.3969/j.issn.1673-5374.2012.17.009

Abstract

In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

Keywords: apoptosis; glucose transporter 3; glucose uptake rate; ischemia; nuclear factor kappaB.