Nanostructured Architectures by Assembling Polysaccharide-Coated BSA Nanoparticles for Biomedical Application

Abstract

Nanostructured architectures are produced on Ti surfaces by layer-by layer (LbL) self-assembling of polysaccharide-coated BSA nanoparticles (BNPs), which created cellular microenvironments mimicking natural extracellular matrix. The BMP-2 encapsulated BNPs are prepared by a desolvation method, and are further coated by chitosan (CHI) coatings to obtain positively charged NPs (CBNPs). Vancomycin (Van) encapsulated CBNPs are obtained by the same method and subsequently coated by oxidized alginate (OALG) to obtain negatively charged NPs (OCBNPs). The CBNPs and OCBNPs are assembled on Ti surfaces to construct nanostructured coatings via electrostatic and covalent interactions. The nanostructured architectures realize the sustained release of BMP-2 and Van for a long term. Bone marrow stromal cells (BMSCs) culture tests confirm that the bare nanostructured architectures intrinsically facilitate attachment, proliferation, and differentiation of cells, which is attributed to the nanoscale porous structures that are similar to the size of cellular filopodia. Incorporating BMP-2 into the nanostructured architectures significantly enhances osteogenetic differentiation of BMSCs, which reveals the synergistic effects of nanostructures and growth factors on cell activity. The antibacterial tests indicate that controlled release of Van has good antibacterial ability against Staphylococcus epidermidis, while not affecting the normal biological activity of BMSCs.

Keywords: bovine serum albumin; dual delivery; nanoparticles; nanostructured architectures; vancomycin.