Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Shuping Nie; Baoyu Tian; Xiaowei Wang; David H Pincus; Martin Welker; Kathleen Gilhuley; Xuedong Lu; Yiping W Han; Yi-Wei Tang

doi:10.1128/JCM.00239-15

Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry

J Clin Microbiol. 2015 Apr;53(4):1399-402. doi: 10.1128/JCM.00239-15. Epub 2015 Feb 4.

Authors

Shuping Nie¹, Baoyu Tian², Xiaowei Wang², David H Pincus³, Martin Welker⁴, Kathleen Gilhuley⁵, Xuedong Lu⁶, Yiping W Han⁷, Yi-Wei Tang⁸

Affiliations

¹ Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
² Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA.
³ bioMérieux, Inc., Hazelwood, Missouri, USA.
⁴ bioMérieux SA, La Balme les Grottes, France.
⁵ Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
⁶ Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China.
⁷ Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA Department of Reproductive Biology, Case Western Reserve University, Cleveland, Ohio, USA ywh2102@columbia.edu tangy@mskcc.org.
⁸ Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York, USA ywh2102@columbia.edu tangy@mskcc.org.

Abstract

We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques*
Fusobacterium Infections / microbiology
Fusobacterium nucleatum / classification*
Fusobacterium nucleatum / genetics*
Humans
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

RNA, Bacterial
RNA, Ribosomal, 16S

Abstract

Publication types

MeSH terms

Substances

Grants and funding