Abstract
XopDXcc8004, a type III effector of Xanthomonas campestris pv. campestris (Xcc) 8004, is considered a shorter version of the XopD, which lacks the N-terminal domain. To understand the functions of XopDXcc8004, in planta, a transgenic approach combined with inducible promoter to analyze the effects of XopDXcc8004 in Arabidopsis was done. Here, the expression of XopDXcc8004, in Arabidopsis elicited the accumulation of host defense-response genes. These molecular changes were dependent on salicylic acid and correlated with lesion-mimic phenotypes observed in XVE::XopDXcc8004 transgenic plants. Moreover, XopDXcc8004 was able to desumoylate HFR1, a basic helix-loop-helix transcription factor involved in photomorphogenesis, through SUMO protease activity. Interestingly, the hfr1-201 mutant increased the expression of host defense-response genes and displayed a resistance phenotype to Xcc8004. These data suggest that HFR1 is involved in plant innate immunity and is potentially regulated by XopDXcc8004.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics*
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Arabidopsis / immunology
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Arabidopsis / microbiology
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / immunology*
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Bacterial Proteins / genetics*
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Bacterial Proteins / immunology
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / immunology*
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Gene Expression Regulation, Plant*
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Host-Pathogen Interactions*
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Mutation
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Nuclear Proteins / genetics
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Nuclear Proteins / immunology*
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Plant Diseases / genetics*
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Plant Diseases / immunology
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Plant Diseases / microbiology
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / immunology
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Salicylic Acid / immunology
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Small Ubiquitin-Related Modifier Proteins / immunology
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Xanthomonas campestris / physiology*
Substances
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Arabidopsis Proteins
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Bacterial Proteins
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DNA-Binding Proteins
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HFR1 protein, Arabidopsis
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Nuclear Proteins
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Small Ubiquitin-Related Modifier Proteins
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Salicylic Acid
Grants and funding
This work was supported by the Ministry of Science and Technology (NSC 100-2321-B- 005-007-MY3, NSC 103-2911-I-005-301, MOST 103-2321-B-005-020) and Ministry of Education (ATU plan), Taiwan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.