MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay

J Microbiol Methods. 2015 Apr:111:21-3. doi: 10.1016/j.mimet.2015.01.024. Epub 2015 Jan 30.

Abstract

MALDI-TOF MS detection of carbapenemase-activity in Gram-negative bacteria was compared against the Carba-NP assay. MALDI-TOF MS detected activity from 99% of the strains, from all types of carbapenemase (200/202), while Carba-NP assays detected activity from 85% (45/53) of the tested isolates and could not consistently identify OXA- or GES carbapenemase activity.

Keywords: Acinetobacter; Carbapenemase detection; Enterobacteriaceae; MALDI-TOF; Pseudomonas.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / enzymology*
  • Bacterial Proteins / metabolism*
  • Bacteriological Techniques
  • Enterobacteriaceae / enzymology*
  • Mass Spectrometry
  • Pseudomonas aeruginosa / enzymology*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase