Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave-assisted fluorescence in situ hybridization

Hematol Oncol. 2017 Mar;35(1):94-100. doi: 10.1002/hon.2194. Epub 2015 Feb 2.

Abstract

Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RARα genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML-RARα rearrangement, which gives clear genetic results within 30 min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright © 2015 John Wiley & Sons, Ltd.

Keywords: acute promyelocytic leukaemia; fluorescence in situ hybridization; microwave oven.

MeSH terms

  • Chromosomes, Human, Pair 15*
  • Chromosomes, Human, Pair 17*
  • DNA Probes
  • DNA, Neoplasm / analysis
  • Female
  • Gene Rearrangement
  • Humans
  • Immunophenotyping
  • In Situ Hybridization
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Leukemia, Promyelocytic, Acute / genetics*
  • Male
  • Microwaves
  • Oncogene Proteins, Fusion / genetics*
  • Translocation, Genetic*

Substances

  • DNA Probes
  • DNA, Neoplasm
  • Oncogene Proteins, Fusion
  • promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein