Tunable nanostructured coating for the capture and selective release of viable circulating tumor cells

Adv Mater. 2015 Mar 4;27(9):1593-9. doi: 10.1002/adma.201404677. Epub 2015 Jan 15.

Abstract

A layer-by-layer gelatin nanocoating is presented for use as a tunable, dual response biomaterial for the capture and release of circulating tumor cells (CTCs) from cancer patient blood. The entire nanocoating can be dissolved from the surface of microfluidic devices through biologically compatible temperature shifts. Alternatively, individual CTCs can be released through locally applied mechanical stress.

Keywords: circulating tumor cells; circulating tumor clusters; microfluidics; nanostructured coatings; single cell sequencing.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / chemistry
  • Biopsy, Fine-Needle
  • Breast Neoplasms / blood
  • Breast Neoplasms / genetics
  • Cell Line, Tumor
  • Cell Separation / instrumentation*
  • Cell Separation / methods
  • Cell Survival
  • Equipment Design
  • Gelatin / chemistry
  • Humans
  • Lung Neoplasms / blood
  • Lung Neoplasms / genetics
  • Male
  • Materials Testing
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Models, Theoretical
  • Nanostructures* / chemistry
  • Neoplastic Cells, Circulating* / chemistry
  • Prostatic Neoplasms / blood
  • Single-Cell Analysis / instrumentation
  • Single-Cell Analysis / methods
  • Stress, Mechanical
  • Temperature

Substances

  • Biocompatible Materials
  • Gelatin