Metabolic profiling by (1)H NMR of ground beef irradiated at different irradiation doses

Meat Sci. 2015 May:103:83-9. doi: 10.1016/j.meatsci.2015.01.005. Epub 2015 Jan 15.

Abstract

This work describes a metabolic profiling study of non-irradiated and irradiated beef (at 2.5, 4.5 and 8 kGy) using (1)H NMR and chemometrics. The assignment of all major NMR signals of the aqueous/methanolic extracts was performed. A comprehensive multivariate data analysis proved the ability to distinguish between the irradiated and non-irradiated beef. Classification trees revealed that three metabolites (glycerol, lactic acid esters and tyramine or a p-substituted phenolic compound) are important biomarkers for classification of the irradiated and non-irradiated beef samples. Overall, the achieved metabolomic results show that the changes in the metabolic profile of meat provide a valuable insight to be used in detecting irradiated beef. The use of the NMR-based approach simplifies sample preparation and decrease the time required for analysis, compared to available official analytical procedures.

Keywords: Beef; Classification trees; Irradiation; Metabolomics; NMR; Principal component analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Esters / analysis
  • Food Analysis / methods*
  • Food Irradiation* / classification
  • Gamma Rays*
  • Glycerol / analysis
  • Humans
  • Lactic Acid / analysis
  • Magnetic Resonance Spectroscopy / methods
  • Metabolome*
  • Metabolomics / methods*
  • Multivariate Analysis
  • Phenols / analysis
  • Red Meat / analysis*
  • Tyramine / analysis

Substances

  • Esters
  • Phenols
  • Lactic Acid
  • Glycerol
  • Tyramine