Transcriptomic analysis of Rongchang pig brains and livers

Ling Gan; Liwei Xie; Fuyuan Zuo; Zhonghuai Xiang; Ningjia He

doi:10.1016/j.gene.2015.01.051

Transcriptomic analysis of Rongchang pig brains and livers

Gene. 2015 Apr 10;560(1):96-106. doi: 10.1016/j.gene.2015.01.051. Epub 2015 Jan 28.

Authors

Ling Gan¹, Liwei Xie², Fuyuan Zuo³, Zhonghuai Xiang⁴, Ningjia He⁵

Affiliations

¹ The Department of Veterinary Medicine, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China. Electronic address: gl9089@sina.com.
² Center of Molecular Medicine, University of Georgia, Athens, GA 30602, USA. Electronic address: liweixie@uga.edu.
³ The Department of Animal Husbandry, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China. Electronic address: zfuyuan@163.com.
⁴ State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. Electronic address: xbxzh@swu.edu.cn.
⁵ State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China. Electronic address: hejia@swu.edu.cn.

PMID: 25637719
DOI: 10.1016/j.gene.2015.01.051

Abstract

Recent developments in high-throughput RNA sequencing (RNA-seq) technology have led to a dramatic impact on our understanding of the structure and expression profiles of the mammalian transcriptome. To gain insights into the usefulness of swine production and biomedical model, the transcriptome profiling of Rongchang pig brains and livers was characterized using RNA-seq technology to uncover functional candidate molecules. In the study, total RNAs from brains and livers of Rongchang pig were sequenced and 8.6Gb sequencing data was obtained. This analysis revealed tissue specificity through the identification of 5575 and 4600 differentially expressed genes (DEGs) in brains and livers, respectively and the functional analysis of DEGs. Furthermore, 83 neuropeptide gene transcripts, 69 neuropeptide receptor gene transcripts, 10 pro-neuropeptide convertase gene transcripts and many other neuropeptide related protein gene transcripts were identified. Totally, the major characteristics of the transcriptional profiles of Rongchang pig brains and livers were present.

Keywords: Neuropeptide; Neuropeptide receptor; Pigs; RNA sequence; Transcriptome profiles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism*
Brain Chemistry
Female
Gene Expression Profiling
Gene Library
High-Throughput Nucleotide Sequencing
Liver / chemistry
Liver / metabolism*
Male
Oligonucleotide Array Sequence Analysis
Sequence Analysis, RNA
Swine / genetics*
Swine / metabolism
Transcriptome*