Abstract
Six new phenolics, scutellariosides A-F (1-3, 5-6, and 8), together with six known compounds (4, 7, 9-12) were isolated from the whole plant of Scutellaria indica (Labiatae). The chemical structures of these compounds were determined by spectroscopic analyses including 2D NMR. Their anti-inflammatory activities were evaluated against LPS-induced NO production in macrophage RAW 264.7 cells. Among them, compounds 10-12 had inhibitory effects with IC50 values ranging from 7.2 to 27.8μM. Compound 12 reduced LPS-induced iNOS expression in a dose-dependent manner.
Keywords:
Anti-inflammatory activity; Flavonoid; Labiatae; RAW 264.7 cells; Scutellaria indica.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Inflammatory Agents / chemistry*
-
Anti-Inflammatory Agents / isolation & purification
-
Anti-Inflammatory Agents / pharmacology*
-
Cell Line
-
Flavonoids / chemistry
-
Flavonoids / isolation & purification
-
Flavonoids / pharmacology
-
Lipopolysaccharides / immunology
-
Macrophages / drug effects*
-
Macrophages / immunology
-
Mice
-
Nitric Oxide / antagonists & inhibitors
-
Nitric Oxide / immunology
-
Nitric Oxide Synthase Type II / antagonists & inhibitors
-
Nitric Oxide Synthase Type II / immunology
-
Phenols / chemistry*
-
Phenols / isolation & purification
-
Phenols / pharmacology*
-
Plant Extracts / chemistry
-
Plant Extracts / isolation & purification
-
Plant Extracts / pharmacology
-
Scutellaria / chemistry*
Substances
-
Anti-Inflammatory Agents
-
Flavonoids
-
Lipopolysaccharides
-
Phenols
-
Plant Extracts
-
Nitric Oxide
-
Nitric Oxide Synthase Type II
-
Nos2 protein, mouse