Synthesis of triazole-nucleoside phosphoramidites and their use in solid-phase oligonucleotide synthesis

Abstract

Triazole-backbone oligonucleotides are macromolecules that have one or more triazole units that are acting as a backbone mimic. Triazoles within the backbone have been used within oligonucleotides for a variety of applications. This unit describes the preparation and synthesis of two triazole-nucleoside phosphoramidites [uracil-triazole-uracil (UtU) and cytosine-triazole-uracil (CtU)] based on a PNA-like scaffold, and their incorporation within oligonucleotides.

Keywords: cycloaddition; phosphoramidite; solid‐phase oligonucleotide synthesis; triazole‐linkage.