Electrochemical determination of the anticancer drug taxol at a ds-DNA modified pencil-graphite electrode and its application as a label-free electrochemical biosensor

Somayeh Tajik; Mohammad Ali Taher; Hadi Beitollahi; Mosoud Torkzadeh-Mahani

doi:10.1016/j.talanta.2014.10.063

Electrochemical determination of the anticancer drug taxol at a ds-DNA modified pencil-graphite electrode and its application as a label-free electrochemical biosensor

Talanta. 2015 Mar:134:60-64. doi: 10.1016/j.talanta.2014.10.063. Epub 2014 Nov 10.

Authors

Somayeh Tajik¹, Mohammad Ali Taher², Hadi Beitollahi³, Mosoud Torkzadeh-Mahani⁴

Affiliations

¹ Department of Chemistry, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran; Young Researchers Society, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran.
² Department of Chemistry, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran.
³ Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: h.beitollahi@yahoo.com.
⁴ Biotechnology Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

PMID: 25618641
DOI: 10.1016/j.talanta.2014.10.063

Abstract

In this study a novel biosensor for determination of taxol is described. The interaction of taxol with salmon-sperm double-stranded DNA (ds-DNA) based on the decreasing of the oxidation signals of guanine and adenine bases was studied electrochemically with a pencil-graphite electrode (PGE) using a differential pulse voltammetry (DPV) method. The decreases in the intensity of the guanine and adenine oxidation signals after interaction with taxol were used as indicator signals for the sensitive determination of taxol. DPV exhibits a linear dynamic range of 2.0×10(-7)-1.0×10(-5) M for taxol with a detection limit of 8.0×10(-8) M. Finally, this modified electrode was used for determination of taxol in some real samples.

Keywords: Anticancer drug determination; DNA biosensor; Double-stranded DNA; Pencil-graphite electrode; Taxol.

MeSH terms

Adenine / chemistry
Antineoplastic Agents, Phytogenic / analysis*
Antineoplastic Agents, Phytogenic / blood
Antineoplastic Agents, Phytogenic / chemistry
Antineoplastic Agents, Phytogenic / urine
Biosensing Techniques*
DNA / chemistry*
Electrochemical Techniques
Electrodes
Graphite
Guanine / chemistry
Humans
Oxidation-Reduction
Paclitaxel / analysis*
Paclitaxel / blood
Paclitaxel / chemistry
Paclitaxel / urine

Substances

Antineoplastic Agents, Phytogenic
Guanine
Graphite
DNA
Adenine
Paclitaxel