High-speed multi-wavelength Fresnel diffraction imaging

Opt Express. 2014 Dec 15;22(25):30504-11. doi: 10.1364/OE.22.030504.

Abstract

We demonstrate a compact lensless microscope which can capture video-rate phase contrast images of moving objects and allows numerical scanning of the focal distance after recording. Using only an RGB-detector and illumination from a single mode fiber, diffraction patterns at three wavelengths are recorded simultaneously, enabling high-speed data collection and reconstruction of phase and amplitude. The technique is used for imaging of a moving test target, beads in a flow cell, and imaging of Caenorhabditis elegans moving in a droplet of liquid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Holography / methods*
  • Lighting*
  • Microscopy, Phase-Contrast / methods*