In this study, we fully assembled eight tomato chloroplast (cp) genomes using next-generation sequencing data. It will provide valuable information for phylogenetic and cp genetic engineering studies of Solanum genus. The total genome size varied from 155,443 to 155,561 bp in length, containing a pair of inverted repeats (IRs) of 25,612-25,639 bp, which were separated by large single copy (LSC) and small single copy (SSC) of 85,857-85,911 bp and 18,362-18,387 bp in size, respectively. The overall GC contents of the chloroplast genome were 37.84-37.88%. The same set of 112 unique genes was annotated from eight cp genomes, including 78 protein coding genes, 30 tRNA genes, and four rRNA genes. Among these, 17 are duplicated in the inverted repeat regions, 15 genes contained one intron, and three genes (rps12, clpP, and ycf3) comprising two introns.
Keywords: Chloroplast genome; Solanum; next-generation sequencing; tomato.