Ultrasonic and free-radical degradation of mannan from Candida albicans

Alžbeta Čížová; Peter Bystrický; Slavomír Bystrický

doi:10.1016/j.ijbiomac.2014.12.046

Ultrasonic and free-radical degradation of mannan from Candida albicans

Int J Biol Macromol. 2015 Apr:75:32-6. doi: 10.1016/j.ijbiomac.2014.12.046. Epub 2015 Jan 17.

Authors

Alžbeta Čížová¹, Peter Bystrický², Slavomír Bystrický²

Affiliations

¹ Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: alzbeta.cizova@savba.sk.
² Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic.

PMID: 25603147
DOI: 10.1016/j.ijbiomac.2014.12.046

Abstract

Mannan from pathogenic Candida albicans serotype A was degraded by means of ultrasound and/or OH generated in situ by Fenton reaction. The kinetics of degradation was monitored by HPLC analysis and the weight-average molecular weights (Mw) and index of polydispersity (PDI) were compared. A well-defined low-molecular-weight mannan (∼ 30 kDa) with narrowed PDI of 1.8 was obtained after 120 min of ultrasonication. Similar or even lower Mw (up to 16 kDa) was achieved upon free-radical exposure depending on Fe(2+) concentration used; however, this was accompanied by overall broadening of PDI and distinct changes in polymer structure as indicated by NMR analysis.

Keywords: Candida albicans; Degradation; Fenton reaction; Mannan; Ultrasound.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Candida albicans / chemistry*
Carbon-13 Magnetic Resonance Spectroscopy
Free Radicals / chemistry*
Kinetics
Mannans / chemistry*
Proton Magnetic Resonance Spectroscopy
Time Factors
Ultrasonics*

Substances

Free Radicals
Mannans