Isolation of isochlorogenic acid isomers in flower buds of Lonicera japonica by high-speed counter-current chromatography and preparative high performance liquid chromatography

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Feb 15:981-982:27-32. doi: 10.1016/j.jchromb.2014.12.020. Epub 2015 Jan 3.

Abstract

An efficient method was established by HSCCC combined with prep-HPLC for separating isochlorogenic acid isomers from flower buds of Lonicera japonica. The partially purified sample from the methanol extract of flower buds of L. japonica by silica gel column was separated by HSCCC to result in a fraction containing two isochlorogenic acid isomers. The fraction was further isolated by prep-HPLC to yield isochlorogenic acid A and isochlorogenic acid C with purities of 98% and 96%, and the total recoveries at 80.1% and 79.8%, respectively. The chemical structures of isochlorogenic acid isomers were confirmed by electrospray ionization mass spectrometry (ESI-MS) and (1)H nuclear magnetic resonance (NMR).

Keywords: Flower buds of Lonicera japonica; High-speed counter-current chromatography; Isochlorogenic acid A; Isochlorogenic acid C; Preparative high performance liquid chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorogenic Acid / analogs & derivatives*
  • Chlorogenic Acid / chemistry
  • Chlorogenic Acid / isolation & purification
  • Chromatography, High Pressure Liquid / methods*
  • Countercurrent Distribution / methods*
  • Flowers / chemistry*
  • Isomerism
  • Lonicera / chemistry*
  • Magnetic Resonance Spectroscopy
  • Plant Extracts / chemistry
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Plant Extracts
  • isochlorogenic acid
  • Chlorogenic Acid