Application of the combination index integrated with confidence intervals to study the toxicological interactions of antibiotics and pesticides in Vibrio qinghaiensis sp.-Q67

Environ Toxicol Pharmacol. 2015 Jan;39(1):447-56. doi: 10.1016/j.etap.2014.12.013. Epub 2014 Dec 29.

Abstract

It is necessary to explore the effect of confidence intervals on the combination index (CI) so that rationally evaluate the toxicological interaction (synergism or antagonism) which is dependent on the concentration ratio, the mixture concentration and the exposure time. To effectively detect the toxicological interaction taking place in mixtures, we combined the CI with the observation-based confidence intervals (OCI) which can characterize the uncertainty in toxicity test and in data fitting. In time scale, the short-term (15min) and long-term (12h) toxicities of three chemicals (imidacloprid (IMI), pirimicarb (PIR) and streptomycin sulfate (STR)) and their binary mixtures on Vibrio qinghaiensis sp.-Q67 were determined by the microplate toxicity analysis (MTA). The mixtures of IMI, PIR and STR have additive actions all but four IMI-PIR rays (R2-R5) at the effect levels above about 30-40% whose long-term toxicological interaction are synergism.

Keywords: Direct equipartition ray design; Long-term toxicity; Microplate toxicity analysis; Short-term toxicity; Synergism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / toxicity*
  • Carbamates / toxicity*
  • Drug Interactions
  • Imidazoles / toxicity*
  • Neonicotinoids
  • Nitro Compounds / toxicity*
  • Pesticides / toxicity*
  • Pyrimidines / toxicity*
  • Streptomycin / toxicity*
  • Vibrio / drug effects*
  • Vibrio / growth & development

Substances

  • Anti-Bacterial Agents
  • Carbamates
  • Imidazoles
  • Neonicotinoids
  • Nitro Compounds
  • Pesticides
  • Pyrimidines
  • pirimicarb
  • imidacloprid
  • Streptomycin