An experiment was conducted to determine the efficacy of Se from hydroponically produced Se-enriched kale sprout (HPSeKS) on performance, carcass characteristics, tissue Se concentration, and physiological responses of broilers in comparison to that of Se from Se-enriched yeast and sodium selenite. Three hundred and sixty male broilers, 10 days of age, were assigned to 6 groups, 4 replicates of 15 broilers each, according to the completely randomized design. The dietary treatments were the following: T1: control diet; T2: control diet plus 0.3 mg Se/kg from sodium selenite; T3: control diet plus 0.3 mg Se/kg from Se-enriched yeast; and T4, T5, and T6: control diet plus 0.3, 1.0, and 2.0 mg Se/kg from HPSeKS, respectively. The results found that dietary Se supplementation did not (p > 0.05) alter performance and carcass characteristics of broilers. Se supplementation increased (p < 0.05) Se concentrations in the liver and kidney of broilers. Heart tissue Se concentration of broilers fed Se from sodium selenite was lower (p < 0.05) than that of broilers fed Se from HPSeKS and Se-enriched yeast. Selenium from HPSeKS increased higher (p < 0.05) GSH-Px activity when compared to Se from sodium selenite and Se-enriched yeast. The results indicated that the efficacy of Se from HPSeKS was comparable in increasing tissue Se concentration, but higher in improving GSH-Px activity in Rbc when compared to those of Se from Se-enriched yeast.