Spatial neuroproteomics using imaging mass spectrometry

Jörg Hanrieder; Per Malmberg; Andrew G Ewing

doi:10.1016/j.bbapap.2014.12.026

Spatial neuroproteomics using imaging mass spectrometry

Biochim Biophys Acta. 2015 Jul;1854(7):718-31. doi: 10.1016/j.bbapap.2014.12.026. Epub 2015 Jan 9.

Authors

Jörg Hanrieder¹, Per Malmberg², Andrew G Ewing³

Affiliations

¹ National Center for Imaging Mass Spectrometry, University of Gothenburg and Chalmers University of Technology, Gothenburg, Sweden; Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden; Institute of Neuroscience and Physiology, Department Psychiatry and Neurochemistry, University of Gothenburg, Sahlgrenska University Hospital Mölndal, Mölndal, Sweden.
² National Center for Imaging Mass Spectrometry, University of Gothenburg and Chalmers University of Technology, Gothenburg, Sweden; Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
³ National Center for Imaging Mass Spectrometry, University of Gothenburg and Chalmers University of Technology, Gothenburg, Sweden; Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. Electronic address: Andrew.ewing@chem.gu.se.

PMID: 25582083
DOI: 10.1016/j.bbapap.2014.12.026

Abstract

The nervous system constitutes arguably the most complicated and least understood cellular network in the human body. This consequently manifests itself in the fact that the molecular bases of neurodegenerative diseases remain unknown. The limited understanding of neurobiological mechanisms relates directly to the lack of appropriate bioanalytical technologies that allow highly resolved, sensitive, specific and comprehensive molecular imaging in complex biological matrices. Imaging mass spectrometry (IMS) is an emerging technique for molecular imaging. The technique is characterized by its high chemical specificity allowing comprehensive, spatial protein and peptide profiling in situ. Imaging MS represents therefore a powerful approach for investigation of spatio-temporal protein and peptide regulations in CNS derived tissue and cells. This review aims to provide a concise overview of major developments and applications concerning imaging mass spectrometry based protein and peptide profiling in neurobiological and biomedical research. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology.

Keywords: Central nervous system (CNS); Imaging mass spectrometry; MALDI-ToF; Peptidomics; Proteomics; ToF-SIMS.

Publication types

Review

MeSH terms

Animals
Humans
Mass Spectrometry / methods*
Nerve Tissue Proteins / metabolism
Neuroimaging / methods*
Proteomics / methods*

Substances

Nerve Tissue Proteins